Comparison of the Effect of Subcutaneous Administration of Anise Essential Oil and Oral Administration of Anise Aqueous Extract in BALB/c Mice Contaminated with Listeriosis

Authors

• Amin Hashempour ¹
• Mohammad Hosein Sadeghi Zali ²
• Saber Yousefi ³

¹ Young Researcher and Elites club, Urmia Branch, Islamic Azad University, Urmia, Iran

² Department of Bacteriology, Veterinary Faculty, Islamic Azad University, Urmia Branch, Urmia, Iran

³ Department of Microbiology, Virology and Immunology, Urmia University of Medical Sciences, Urmia, Iran

Abstract

Introduction: Studies have shown antibacterial activity of anise and on the other hand, Listeria monocytogenes, one Gram-positive pathogen is responsible for listeriosis. Thus, this study aimed to investigate the effect of subcutaneous administration of anise essential oil and oral administration of its aqueous extract and also comparison of their effects in BALB/c mice infected with listeriosis. Methods: Two units, by using insulin syringe, of Listeria monocytogenes prepared in 0.5 McFarland standard was intraperitoneally administrated to mice and agglutination test was done to ensure before anise administration. BALB/c mice (n=50) infected with listeriosis received anise aqueous extract (AAE) (5 g/100 g drink water) and 1-unit anise essential oil (AEO) by using insulin syringe was subcutaneously administrated to other mice (n=50) for 1 week. Other mice (n=24) were considered as control. Results: Results showed that AAE and AEO could improve mice contaminated with listeriosis, although mice treated with AAE were efficiently cured than those who received AEO (37 vs. 12). Conclusion: On the basis of the results, it can be recommended that the use of AAE for treatment of listeriosis is tenable.

Keywords

• Agglutination Test
• Anise Aqueous Extract
• Antibacterial Activity
• Listeriosis

---

References

1. Vazquez- Bolland JA, Kuhn M, Berche P, Chakraborty T, Dominquez- Bernal G, Goebel W, et al. Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev. 2001; 14: 584- 640.

2. Farber JM, Peterkin PI. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev. 1991; 55: 476- 511.

3. North RJ, Dunn PL, Conlan JW. Murine listeriosis as a model of antimicrobial defense. Immunol Rev. 1997; 158: 27- 36.

4. Mbandi E, Shelef LA. Enhanced antimicrobial effects of combination of lactate and diacetate on Listeria monocytogenes and Salmonella spp. in beef bologna. Int J Food Microbiol. 2002; 76: 191- 198.

5. Vogel BF, Hansen LT, Mordhorst H, Gram L. The survival of Listeria monocytogenes during long term desiccation is facilitated by sodium chloride and organic material. Int J Food Microbiol. 2010; 140: 192- 200.

6. Xie TP, Zhao YF, Chen LQ, Zhu ZJ, Hu Y, Yuan Y. Long-term exposure to sodium nitrite and risk of esophageal carcinoma: a cohort study for 30 years. Dis Esophagus. 2011; 24: 30- 32.

7. Michelet C, Avril FC, Berche P. Inhibition of intracellular growth of Listeria monocytogenes by antibiotics. Antimicrob. Agents Chemother. 1994; 38: 438- 446.

8. Lorber B. Listeriosis. Clin Infect Dis. 1997; 24: 1- 11.

9. McFarland LV. Epidemiology, risk factors and treatments for antibiotic-associated diarrhea. Dig Dis. 1998; 16: 292- 307.

10. Jernberg C, Lofmark S, Edlund C, Jansson JK. Long-term impacts of antibiotic exposure on the human intestinal microbiota. Microbiology. 2010; 156: 3216- 3223.

11. Kurek A, Grudniak AM, Szwed M, Klicka A, Samluk L, Wolska KI, Janiszowska W, Popowska M. Oleanolic acid and ursolic acid affect peptidoglycan metabolism in Listeria monocytogenes. Antonie van Leewenhoek. 2010; 97: 61- 68.

12. Singab AN, Ayoub NA, Ali EN, Mostafa NM. Antioxidant and hepatoprotective activities of Egyptian moraceous plants against carbon tetrachloride-induced oxidative stress and liver damage in rats. Pharm Biol. 2010; 48: 1255- 1264.

13. Ross IA. Medicinal plants of the world. Chem Constitutes. 2001; 2: 363- 374.

14. Tirapelli CR, Andrade CR, de Cassano AO, de Souza FA, Ambrosio SR, Costa FB, da Oliveria AM. Antispasmodic and relaxant effects of the hydroalcoholic extract of Pimpinella anisum (Apiaceae) on rat anococcygeous smooth muscle. J Enthopharmacol. 2007; 110 (1): 23- 29.

15. Özcan MM, Chalchat JC. Chemical composition and antifungal effect of anise (Pimpinella anisum L.) fruit oil at ripening stage. Ann Microbiol. 2006; 56 (4): 353- 358.

16. Akhtar AA, Deshmukh AV, In vitro Antibacterial activity of Pimpinella anisum fruit extracts against some pathogenic bacteria. Veterinary World. 2008; 1 (9): 272- 274.

17. Ates DA, Erdogrul OT. Antimicrobial activities of various medicinal and commercial plant extracts. Turkish J Biol. 2003; 27: 157- 162.

18. McFarland J. Nephelometer: an instrument for media used for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. J Am Med Assoc. 1907; 14: 1176- 1178.

19. Rodrigues VM, Rosa PTV, Marques MOM, Petenale AS, Meireles MAA. Supercriticial extraction of essential oil from aniseed (Pimpinella anisum L.) using CO2 solubility, kinetics and composition data. J Agric Food Chem. 2003; 51: 1518- 1523.

20. Tabanca N, Demirci B, Kirimer N, Baser KHC, Bedir E, Khan IA, Wedge DE. Gas chromatographic-Mass spectrometric analysis of essential oil from Pimpinella species gathered from Central and Northern turkey. J Chromatogr. 2006; 1117: 194- 205.

21. Huxley A. The new RHS dictionary of gardening.4 ed. Paper and slipcase edition, United Kingdom. 1992.

22. Gülcin I, Oktay M, Kirecci E, Küfrevioglu ÖI. Screening of antioxidant and antimicrobial activities of anise Pimpinella anisum L. seed extracts. Food Chem. 2003; 83: 371- 382.

23. Mahady GB, Pendland SL, Stoia A, Hamill FA, Fabricant D, Dietz BM, Chadwick LR. In vitro susceptibility of helicobacter pylori to botanical extracts used traditionally for the treatment of gastrointestinal disorders. Phototherapy Res. 2005; 19 (11): 988- 991.