The Effect of Concurrent Endurance and Resistance Exercise on Plasma Levels of Vascular Endothelial Growth Factor and Endostatin in Inactive Women

Document Type : Original Article

Authors

• Saeedeh Shadmehri
• Mozhgan Ahmadi

Department of Physical Education and Sport Sciences, Yadegar-e-Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction: The present study aims to investigate the effect of one concurrent endurance and resistance exercise training session on plasma levels of vascular endothelial growth factor (VEGF) and endostatin in inactive women. Methods: In this quasi-experimental study, 12 inactive female university students were divided into control (average age=20.0 ±1.78 years, height = 161.3 ±50 cm, weight = 58.83±5.71 kg, BMI = 22.38±3.23 kg/m2) and exercise (average age =19.5±1.04 years, height = 163.1±3.79 cm, weight = 58.67±7.69 kg, BMI =22.35±2.22 kg/m2) groups. The endurance exercise was performed on a treadmill with 65% of MHR for 16 minutes. The resistance exercises of four movements including leg press, chest compression, armpit and leg stretch were performed at 50% 1RM in two sets with 10 repetitions and 1-2 minutes’ rest intervals between sets. Data were analyzed using dependent t-test and independent t-test to determine differences within the group and between-group at pthe control group (p= 0.168). Conclusion: According to the findings, it seems that one session of concurrent endurance and resistance exercise has a positive influence on VEGF and probably it can help to improve the condition of angiogenesis.

Keywords

• Vascular Endothelial Growth Factor
• Endostatin
• Concurrent Endurance and Resistance Exercise

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References

1. Olfert IM, Baum O, Hellsten Y, Egginton S. Advances and challenges in skeletal

muscle angiogenesis. Am J Physiol Heart Circ Physiol. 2016; 310 (3): 326- 336.

2. Jensen L, Bangsbo J, Hellsten Y. Effect of high intensity training on capillarization and presence of angiogenic factors in human skeletal muscle. J Physiol. 2004; 557 (2): 571- 582.

3. Tesch PA, Thorsson A, Kaiser P. Muscle capillary supply and fiber type characteristics in weight and power lifters. J Appl Physiol. 1984; 56: 35- 38.

4. Bruserud O, Grovan F, Lindås R, Blymke Møinichen C, Osterhus KK. Serum levels of angioregulatory mediators in healthy individuals depend on age and physical activity: studies of angiogenin, basic fibroblast growth factor, leptin and endostatin. Scand J Clin Lab Invest. 2006; 65 (6): 505- 511.

5. Gabrilove JL. Angiogenic growth factors: autocrine and paracrine regulation of survival in hematologic malignancies. Oncologist. 2001; 5: 4- 7.

6. Stammers R, Robinson CJ, Forster NM, Wagner B, Rafferty B. Vascular endothelial growth factor (VEGF): modulation of heparin-binding activity and bioactivity by site-directed mutagenesis. Endocrine. 2005; 9: 10- 27.

7. Tang K, Breen EC, Gerber HP, Ferrara NM, Wagner PD. Capillary regression in vascular endothelial growth factor-deficient skeletal muscle. Physiol Genomics. 2004; 18: 63- 69.

8. Gavin TP, Ruster RS, Carrithers JA, Zwetsloot KA, Kraus RM, Evans CA, et al. No difference in the skeletal muscle angiogenic response to aerobic exercise training between young and aged men. J Physiol. 2007; 585 (1): 231- 239.

9. Ranjit PM, Anuradha C, Vishnupriya S, Girijasankar G, Girish K, Chowdary Y. Endogenous angiogenesis inhibitor endostatin: An Overview. Liver J. 2012; 13- 24.

10. Vasiliadis AV, Zafeiridis A, Dipla K, Galanis N, Chatzidimitriou D, Kyparos A, et al. Circulating angiogenic biomolecules at rest and in response to upper-limb exercise in individuals with spinal cord injury. J Spinal Cord Med. 2014; 37 (2): 226- 232

11. Hiscock N, Fischer CP, Pilegaard H, Pedersen BK. Vascular endothelial growth factor mRNA expression and arteriovenous balance in response to prolonged, submaximal exercise in humans. Am J Physiol Heart Circ Physiol. 2003; 285: 1759- 1763.

12. Gavin TP, Robinson CB, Yeager RC, England JA, Nifong LW, Hickner RC. Angiogenic growth factor response to acute systemic exercise in human skeletal muscle. J Appl Physiol. 2004; 96: 19- 24.

13. Gavin T, Drew J, Kubik C, Pofahl W, Hickner R. Acute resistance exercise increases skeletal muscle angiogenic growth factor expression. Acta Physiol. 2007; 191 (2): 139- 146.

14. Kraus RM, Stallings WH, Yeager RC, Gavin TP. Circulating plasma VEGF response to exercise in sedentary and endurance-trained men. J Appl Physiol. 2004; 96: 1445- 1450.

15. Khazaei M, Salehi E, Rashidi B. Pan PPAR agonist, bezafibrate, restores angiogenesis in hindlimb ischemia in normal and diabetic rats. Int J Pept. 2012; 6372- 6382.

16. Gu J, Gadonski G, Wang J, Makey I, Adair T. Exercise increases endostatin in circulation of healthy volunteers. BMC Physiol. 2004; 4 (2): 1- 7.

17. Tolouei Azar J, Ravasi A, Soori R, Akbarnejad A, Hemati Nafar M. The effect of 8 weeks aerobic training on angiogenesis (VEGF) and angiostatic (ES) factors in sedentary women. J Urmia Univ Med Sci. 2016; 27 (12): 1032- 1040.

18. Nourshahi M, Hedayati M, Nemati J, Ranjbar K, Gholamali M. Effect of 8 weeks endurance training on serum vascular endothelial growth factor and

endostatin in Wistar rats. Koomesh. 2012; 13 (4): 474- 479.

19. Richardson RS, Knight DR, Poole DC, Kurdak SS, Hogan MC, Grassi B, et al. Determinants of maximal exercise VO2 during single leg knee extensor exercise in humans. Am J Physiol Heart Circ Physiol. 1995; 268: 1453- 1461.

20. Gale NW, Yancopoulos GD. Growth factors acting via endothelial cell-specific receptor tyrosine kinases: VEGFs angiopoietins and ephrins in vascular development. Genes Dev. 1999; 13: 1055- 66.

21. Hosseini M, Piri M, Agha-Alinejad H, Haj-Sadeghi Sh. The effect of endurance, and resistance and concurrent training on the heart structure of female students. Biol Sport. 2012; 29: 17- 21.

22. Van Pelt DW, Guth LM, Horowitz JF. Aerobic exercise elevates markers of angiogenesis and macrophage IL-6 gene expression in the subcutaneous adipose tissue of overweight-to-obese adults. J Appl Physiol. 2016; 123 (5): 1150- 1159.

23. Rullman E, Rundqvist H, Wagsa¨ter D, Fischer H, Eriksson P, Sundberg CJ, et al. A single bout of exercise activates matrix metalloproteinase in human skeletal muscle. J Appl Physiol. 2007; 102: 2346- 2351.

24. Suzuki J. L- arginine supplementation causes additional effects on exercise- induced angiogenesis and VEGF expression in the heart and hind- leg muscles of middle aged rats. J Physiol Sci. 2006; 56: 39- 44.

25. Hoffner L, Nielsen JJ, Langberg H, Hellsten Y. Exercise but not prostanoids enhance levels of vascular endothelial growth factor and other proliferative agents in human skeletal muscle interstitium. J Physiol. 2003; 550: 217- 225.

26. Nader GA. Concurrent strength and endurance training: from molecules to man. Med Sci Sports Exerc. 2006; 38: 1965- 1970.

27. Takahashi A, Kureishi Y, Yang J, Luo Z, Guo K, Mukhopadhyay D, et al. Myogenic Akt signaling regulates blood vessel recruitment during myofiber growth. Mol Cell Biol. 2002; 22: 4803- 4814.

28. Trenerry MK, Carey KA, Ward AC, Cameron-Smith D. STAT3 signaling is activated in human skeletal muscle following acute resistance exercise. J Appl Physiol. 2007; 102: 1483- 1489.

29. Gustafsson T, Knutsson A, Puntschart A, Kaijser L, Sandberg Nordqvist AC, Sundberg CJ, et al. Increased expression of vascular endothelial growth factor in human skeletal muscle in response to short-term one-legged exercise training. Eur J Physiol. 2002; 444: 752- 759.

30. Seida A, Wada J, Kunitomi M, Tsuchiyama Y, Miyatake N, Fujii M, et al. Serum bFGF levels are reduced in

Japanese overweight men and restored by a 6-month exercise education. Int J Obes Relat Metab Disord. 2003; 27: 1325- 1331.

31. Brixius K, Schoenberger S, Ladage D, Knigge H, Falkowski G, Hellmich M, et al. Long-term endurance exercise decreases antiangiogenic endostatin signaling in overweight men aged 50-60 years. Br J Sports Med. 2008; 42: 126- 129.

32. Suhr F, Rosenwick C, Vassiliadis A, Bloch W, Brixius K. Regulation of extracellular matrix compounds involved in angiogenic processes in short- and long-track elite runners. Scand J Med Sci Sports. 2010; 20: 441- 448.

33. Noris M, Morigi M, Donadelli R, Aiello S, Foppolo M, Todeschini M, et al. Nitric oxide synthesis by cultured endothelial cells is modulated by flow conditions. Circ Res. 1995; 76: 536- 543.