The Responses of Muscle Damage Markers and Growth Mediator to Different Concurrent protocol of Endurance and Resistance Training

Mohamadzadeh Salamat, Khalid

The Responses of Muscle Damage Markers and Growth Mediator to Different Concurrent protocol of Endurance and Resistance Training

Document Type : Original Article

Author

Khalid Mohamadzadeh salamat

Department of Physical Education and Exercise Science, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

Abstract

Introduction: The aim of this study was to investigate the muscle damage markers and growth mediator responses to concurrent training with different endurance and resistance training order in healthy males. Methods: Thirty-nine healthy male were randomly assigned into three equal homogeneous groups; endurance-resistance training (ER), resistance- endurance concurrent training (RE) and control (C). The training group’s subjects performed eight weeks 3sessions per week concurrent training sessions in the same intensity and duration but different by endurance and resistance exercise orders. Tow incremental exhaustive treadmill tests was performed before training and 72 h after the last training session. Blood samples for the measurement of creatine kinase (CK), lactate dehydrogenase (LDH), insulin-like growth factor (IGF)-1 and insulin-like growth factor binding protein (IGFBP)-3 were collected at baseline and immediately after the tow exhaustive treadmill tests. Results: The response of CK, LDH, IGF-1 and IGFBP-3 to the first and second incremental exhaustive treadmill test showed significant increases in both exercise orders when compared to baseline values (p<0.05). Also, comparison of pre and post-training responses showed a significant decrease in CK and LDH in both exercise order and a significant increase in IGFBP-3 and IGF-1 only in the RE exercise order (P<0.05). However, the present study results didn’t show any significant difference between the ER and RE groups. Conclusion: According to the results, there were significant decreases in muscle damage markers after both types of concurrent training. However, higher growth mediator’s responses were seen when resistance exercise precedes endurance exercise.

Keywords

References

1. Coffey VG, Pilegaard H, Garnham AP, O'Brien BJ, Hawley JA. Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle. J Appl Physiol. 2009; 106 (4): 1187- 97.

2. Hawley JA. Molecular responses to strength and endurance training: Are they incompatible?. Appl Physiol Nutr Metab. 2009; 34 (3): 355- 361.

3. Cadore EL, Izquierdo M, dos Santos MG, Martins JB, Lhullier FL, Pinto RS, Silva RF, Kruel LF. Hormonal responses to concurrent strength and endurance training with different exercise orders. J Strength Cond Res. 2012; 26 (12): 3281- 3288.

4. MacNeil LG, Glover E, Bergstra TG, Safdar A, Tarnopolsky MA. The order of exercise during concurrent training for

rehabilitation does not alter acute genetic expression, mitochondrial enzyme activity or improvements in muscle function. PloS one. 2014; 9 (10): e109189.

5. Wilson JM, Marin PJ, Rhea MR, Wilson SM, Loenneke JP, Anderson JC. Concurrent training: a meta-analysis examining interference of aerobic and resistance exercises. J Strength Cond Res. 2012; 26 (8): 2293- 2307.

6. Alkner BA, Tesch PA. Knee extensor and plantar flexor muscle size and function following 90 days of bed rest with or without resistance exercise. Eur J Appl Physiol. 2004; 93 (3): 294- 305.

7. Baumgartner RN, Wayne SJ, Waters DL, Janssen I, Gallagher D, Morley JE. Sarcopenic obesity predicts instrumental activities of daily living disability in the elderly. Obes Res. 2004; 12 (12): 1995- 2004.

8. Izquierdo M, Hakkinen K, Ibanez J, Kraemer WJ, Gorostiaga EM. Effects of combined resistance and cardiovascular training on strength, power, muscle cross-sectional area, and endurance markers in middle-aged men. Eur J Appl Physiol. 2005; 94 (1-2): 70- 75.

9. Allen J, Morelli V. Aging and exercise. Clin Geriatr Med. 2011; 27 (4): 661- 671.

10. Campbell EL, Seynnes OR, Bottinelli R, McPhee JS, Atherton PJ, Jones DA, et al. Skeletal muscle adaptations to physical inactivity and subsequent retraining in young men. Biogerontology. 2013; 14 (3): 247- 259.

11. Rosa C, Vilaca-Alves J, Fernandes HM, Saavedra FJ, Pinto RS, dos Reis VM. Order effects of combined strength and endurance training on testosterone, cortisol, growth hormone, and IGF-1 binding protein 3 in concurrently trained men. J Strength Cond Res. 2015; 29 (1): 74- 79.

12. Salamat KM, Azarbayjani MA, Yusof A, Dehghan F. The response of pre-inflammatory cytokines factors to different exercises (endurance, resistance, concurrent) in overweight men. Alexandria J Med. 2016; 52 (4): 367- 370.

13. Alves AR, Marta CC, Neiva HP, Izquierdo M, Marques MC. Does intrasession concurrent strength and aerobic training order influence training- induced explosive strength and VO2max in prepubescent children?. J Strength Cond Res. 2016; 30 (12): 3267- 3277.

14. Chtara M, Chaouachi A, Levin GT, Chaouachi M, Chamari K, Amri M, Laursen PB. Effect of concurrent endurance and circuit resistance training sequence on muscular strength and power development. J Strength Cond Res. 2008; 22 (4): 1037- 1045.

15. Drummond MJ, Vehrs PR, Schaalje GB, Parcell AC. Aerobic and resistance exercise sequence affects excess postexercise oxygen consumption. J Strength Cond Res. 2005; 19 (2): 332- 337.

16. Kang J, Ratamess N. Which comes first? resistance before aerobic exercise or vice versa?. ACSMs Health Fit J. 2014;18 (1): 1- 8.

17. Schoenfeld BJ. Does exercise-induced muscle damage play a role in skeletal muscle hypertrophy? J Strength Cond Res. 2012; 26 (5): 1441- 1453.

18. Rech A, Radaelli R, De Assis AM, Fernandes JR, Longoni A, Vozari-Hampe MM, et al. The effects of strength, aerobic, and concurrent exercise on skeletal muscle damage in rats. Muscle Nerve. 2014; 50 (1): 79- 86.

19. Clarkson PM, Kearns AK, Rouzier P, Rubin R, Thompson PD. Serum creatine kinase levels and renal function measures

in exertional muscle damage. Med Sci Sports Exerc. 2006; 38 (4): 623- 627.

20. Taipale RS, Häkkinen K. Acute hormonal and force responses to combined strength and endurance loadings in men and women: the “order effect”. PloS one. 2013; 8 (2): e55051.

21. Jones TW, Howatson G, Russell M & French DN. Effects of strength and endurance exercise order on endocrine responses to concurrent training, Eur J Sport Sci. 2017; 17 (3): 1- 9.

22. Enright K, Morton J, Iga J,, Drust B. Hormonal responses during two different concurrent-training trials in youth elite soccer players: does changing the organisation of training impact the hormonal response to concurrent exercise?. J Sports Med Phys Fitness. 2017; 22: 12- 19.

23. Heyward V, Gibson A. Advanced fitness assessment and exercise prescription. 7th Edition ed. United State: Human Kinethic. 2014; 536.

24. Armstrong RB. Muscle damage and endurance events. Sports Med. 1986; 3 (5): 370- 381.

25. Ramos-Campo DJ, Ávila-Gandía V, Alacid F, Soto-Méndez F, Alcaraz PE, López-Román FJ, Rubio-Arias JA. Muscle damage, physiological changes and energy balance in ultra-endurance mountain event athletes. Appl Physiol Nutr Metab. 2016; 7: 14- 21.

26. Fyfe JJ, Bishop DJ, Stepto NK. Interference between concurrent resistance and endurance exercise: molecular bases and the role of individual training variables. Sports Med. 2014; 44 (6): 743- 762.

27. Charge SB, Rudnicki MA. Cellular and molecular regulation of muscle regeneration. Physiol Rev. 2004; 84 (1): 209- 238.