Effect of Zinc Oxide Nano-Particles on Motor Coordination in the Attendance of Vitamin C in Rats

Document Type : Original Article

Authors

• Maryam Rafieirad ¹
• Saeed Valipour Chahardah Charic ²

¹ Department of biology, Izeh branch, Islamic Azad University, Izeh, Iran

² Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran

Abstract

Introduction: Zinc oxide is an inorganic compound with the formula ZnO. Oxide is widely used in various applications, especially in pharmaceutical applications, as well as technical and cosmetic products. The aim of this study is to evaluate the effect of zinc oxide nano-particles (ZnO NPs) on the motor coordination of male rats in the presence and absence of vitamin C as an antioxidant. Methods: In this experimental study, 80 male Wistar rats with a weight range of 200-250 g were used. The rats were randomly divided into ten 8-memebr groups control group (saline receivers) and rats which received zinc nano-oxide with doses 1.25, 2.5 and 5 mg/kg, rats receiving vitamin C with doses 30, 60 and 120 mg/kg and rats which received both zinc nano-oxide with doses 1.25, 2.5 and 5 mg/kg and vitamin C with a dose of 30 mg/kg.while different groups received the above doses intraperitoneally (i.p) the rate of motor coordination was measured in the first day of study using rotarod machine. Motor coordination was considered as the duration a rat could stay on the rotarod. Results: According to the results, dose dependent ZnO NPs with doses 1.25, 2.5 and 5 mg/kg and the simultaneous prescription of different doses of ZnO NPs and a neutral amount of vitamin C decrease this rotarod duration. Conclusion: ZnO NPs disturb motor coordination. Probably, this is not due to the oxidative feature of zinc because vitamin C could not improve this effect. It seems that this effect is applied by another mechanism such as changing the activities of neurochemical system contributing to motor coordination.

Keywords

• ZnO NPs
• Motor Coordination
• Vitamin C
• Rat

---

References

1. Bhatnagar S, Taneja S. Zinc and cognitive development. Br J Nutr. 2001; 85(Suppl 2): S139- 145.

2. Black MM, Sazawal S, Black RE, Khosla S, Kumar J, Menon V. Cognitive and motor development among small-for-gestational-age infants: impact of zinc supplementation, birth weight, and caregiving practices. Pediatrics J. 2004; 113 (5): 1297- 1305.

3. Smart TG, Hosie AM, Miller PS. Zn2+ ions: modulators of excitatory and inhibitory synaptic activity. Neuroscientist J. 2004; 10 (5): 432- 442.

4. Lockman PR, Koziara JM, Mumper RJ, Allen DD. Nanoparticle surface charges alter blood-brain barrier integrity and permeability. J Drug Target. 2004; 12 (9-10): 635- 641.

5. Kocaturk S, Kocaturk PA, Kavas GO, Mutluer N. Antioxidant defence system in a patient with cerebrovascular accident. J Int Med Res. 1996; 24 (4): 376- 380.

6. Sayre LM, Perry G, Smith MA. Oxidative stress and neurotoxicity. Chem Res Toxicol. 2008; 21 (1): 172- 188.

7. Takeda A. Zinc homeostasis and functions of zinc in the brain. Biometals J. 2001; 14 (3-4): 343- 351.

8. Takeda A, Tamano H, Kan F, Hanajima T, Yamada K, Oku N. Enhancement of social isolation-induced aggressive behavior of young mice by zinc deficiency. Life Sci. 2008; 82 (17-18): 909- 914.

9. Duarte TL, Lunec J. Review: When is an antioxidant not an antioxidant? A review of novel actions and reactions of vitamin C. Free Radic Res. 2005; 39 (7): 671- 686.

10. Sartori-Valinotti JC, Iliescu R, Fortepiani LA, Yanes LL, Reckelhoff JF. Sex differences in oxidative stress and the impact on blood pressure control and cardiovascular disease. Int J Clin Exp Physiol. 2007; 34 (9): 938- 945.

11. Li Y, Schellhorn HE. New developments and novel therapeutic perspectives for vitamin C. J Nutr. 2007; 137 (10): 2171- 2184.

12. Kesmati M, Torabi M, Malekshahinia H, Teymuri Zamaneh H. Effect of chronic administration of zinc supplements (ZnO and nano ZnO) with and without aerobic exercise on nociception in male rats. Physiol Pharmacol. 2013; 16 (4): 415- 422.

13. Goudarzi S, Rafieirad M. Evaluating the effect of α-pinene on motor activity, avoidance memory andlipid peroxidation in animal model of Parkinson disease in adult male rats. RJP. 2017; 4 (2): 53- 63.

14. Ghotbeddin Z, Moazedi AA, Parham GA. Effect of combined administration of Zinc chloride and Aluminum chloride on memory and motor activity of young rats. Physiol Pharmacol. 2007; 11 (2): 146- 152.

15. Mocchegiani E, Bertoni-Freddari C, Marcellini F, Malavolta M. Brain, aging and neurodegeneration: role of zinc ion availability. Prog Neurobiol. 2005; 75 (6): 367- 390.