Effect of Six Weeks Light Intensity Interval Training on ANP and BNP Gene Expression Levels after Myocardial Infarction

Document Type : Original Article

Authors

• Mehran Ghahramani ¹
• Sara Karbalaeifar ²

¹ Department of Physical Education, Gilan-E-Gharb, Islamic Azad University, Gilan-E-Gharb,Iran.

² Department of Physical Education, Tehran University, Kish International Campus, Kish, Iran

Abstract

Introduction: Myocardial infarction (MI) is the irreversible cell death caused by ischemia in parts of myocardium. Atrial and brain natriuretic peptides (ANP and BNP) are known as strong markers of myocardial infarction. This research aimed to evaluate the effect of precedent of six-week light intensity interval training (LIIT) on the ANP and BNP gene expression in rats after Myocardial Infarction. Method: Twelve Wistar male rats of 10 weeks old and mean weight 250-300gr were allocated in two groups with six Rats in each groups of 1. LIIT (60 minutes interval running on a treadmill, each cycle including four minutes of running with an intensity of 55-60% VO2max and two minutes of active recovery with a 45-50% VO2max intensity, three days a week for six weeks) and 2. control group (without any precedent of training). Real-time PCR was used to assess the expression of ANP and BNP genes in myocardium (after inducing MI By blocking left coronary artery by surgery). For statistical analysis of data independent sample t test (p≤0.05) was used. Results: Light intensity interval training has significant effect on decreasing the gene expression of ANP (p=0.01) and BNP (p=0.001) in rats after myocardial infarction. Conclusion: It appears that adaptation to light intensity interval training controls and moderates the secretion of cardiac hormones in rats after myocardial infarction.

Keywords

• Myocardial Infarction
• ANP
• BNP
• Light Intensity Interval Training

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References

1. Nathalie M, Benda TM, Eijsvogels AP, Dijk M, Hopman TE, Dick H, Thijssen J. Changes in BNP and cardiac troponin I after high-intensity interval and endurance exercise in heart failure patients and healthy controls. Int J Cardiol. 2015; 1 (184): 426- 427.

2. Krupicka T, Janota Z, Kasalova J. Natriuretic peptides- physiology,

pathophysiology and clinical use in heart failure. Physiol Res. 2009; 58: 171- 177.

3. Durak- Nalbantic A, Dzubur A, Dilic M, Pozderac Z, Mujanovic-Narancic A, Kulic M, et al. Brain natriuretic peptide release in acute myocardial infarction. Bosn J Basic Med Sci. 2012; 12 (3): 164- 168.

4. Karciauskaite D, Grybauskiene R, Grybauskas P, Janenaite J. Brain natriuretic peptide and other cardiac markers in predicting left ventricular remodeling in patients with the first myocardial infarction. Medicina. 2004; (40): 949- 956.

5. Nilsson J, Groenning B, Nielsen G, Fritz-Hansen T, Trawinski J, Hildebrandt P et al. Left ventricular remodeling in the first year after acute myocardial infarction and the predictive value of N- terminal pro brain natriuretic peptide. Am Heart J. 2002; (143): 696- 702.

6. Ahmadizad S, El-Sayed MS. The effects of graded resistance exercise on platelet aggregation and activation. Med Sci Sports Exerc. 2003; (35): 1026- 1033.

7. Fagard RH. Exercise is good for your blood pressure: effects of endurance training and resistance training. Clin Exp Pharmacol Physiol. 2006; (33): 853- 856.

8. Kraemer WJ, Ratamess NA. Fundamentals of resistance training: progression and exercise prescription. Med Sci Sports Exer. 2004; (36): 674- 688.

9. Suda K, Hagiwara H, Kotani Y, Kato K, Sasaki M, Izawa T, et al. Effect of exercise training on ANP receptors. Res Commun Mol Pathol Pharmacol. 2000; (108): 227- 235.

10. Sarullo MF, Gristina T, Brusca I, Milia S, Raimondi R, Sajeva M, et al. Effect of physical training on exercise capacity. gas exchange and N-terminal pro-brain natriuretic peptide levels in patients with chronic heart failure. Eur J Cardiovasc Prev Rehabil. 2006; (13): 812- 817.

11. Kanda T, Takahashi T, Interleukin- 6 and cardiovascular diseases. Jpn Heart J. 2004; (45): 183- 193.

12. Irving H, Zucker Harold D, Schultz Kaushik PP, Hanjun W. Modulation of angiotensin II signaling following exercise training in heart failure. Am J Physiol Heart Circ Physiol. 2015; 308: 781- 791.

13. Ravasi AA, Kordi MR, Naghizade S, Kazemi F. The effect of a session of endurance and sprint exercises on plasma ANP in male athlete students. Harecat J. 2011; 4 (14): 125- 138.

14. Khalighfard S, Gaeini A, Nazarali P. The effect of endurance exercise on cardiac stress and exercise induced immune response in elite kayakers. RJMS. 2011; 17 (80): 8- 15.

15. Thijs M, Eijsvogels H, Arie PJ, Dijk V, Maria TE, Hopman Changes in BNP and cardiac troponin I after high-intensity interval and endurance exercise in heart failure patients and healthy controls. Int J Cardiol. 2015; (184): 426- 427.

16. Guazzi M, Vitelli A, Arena R. The effect of exercise training on plasma NT-pro-BNP levels and its correlation with improved exercise ventilatory efficiency in patients with heart failure. Int J Cardiol. 2012; 2 (158): 290- 299.

17. Morten A, Hoydal MA, Wisloff U, Kemi OJ, Ellingsen O. Running speed and

maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Cardiovasc Prev Rehabil. 2007; 14 (6): 753- 760.

18. Wisloff U, Helgerud J, Kemi OJ, Ellingsen O. Intensity- controlled treadmill running in rats: VO2max and cardiac hypertrophy. Am J Physiol Heart Circ Physiol. 2000; 280 (3): 301- 310.

19. Kraljevic J, Marinovic J, Pravdic D, Zubin P, Dujic Z, Wisloff U, et al. Aerobic interval training attenuates remodelling and mitochondrial dysfunction in the post-infarction failing rat heart. Cardiovasc Res. 2013; 99 (1): 55- 64.

20. Borer KA. Advanced exercise endocrinology. Human Sci Pub. 2013; 204- 241.

21. Ma KK, Ogawa T, De Bold AJ. Selective up regulation of cardiac brain natriuretic peptide at the transcriptional and translational levels by proinflammatory cytokines and by conditioned medium derived from mixed lymphocyte reactions via p38 MAP kinase. J Mol Cell Cardiol. 2004; (36): 505- 513.

22. Conraads V, Beckers P, Vaes J, Martin M, Van Hoof V, De Maeyer C, et al. Combined endurance/ resistance training reduces NT- proBNP levels in patients with chronic heart failure. Eur Heart J. 2004; (25): 1797- 1805.