The Effect of One Session of Exhaustive Training on Some Biochemical Markers of Skeletal Muscles and Hepatic Metabolism in Men Handball Players

Document Type : Original Article

Authors

1 Department of Physical Education, Mahallat Branch, Islamic Azad University, Mahallat, Iran

2 Trauma Research Center, College of Medicine, Kashan University of Medical Sciences, Kashan, Iran

Abstract

Introduction: Evidence has shown that an increase in the skeletal and hepatic biochemical markers is one of the main factors in the exhaustion of the individual. The aim of this study was to investigate the effect of one session of exhaustive training on some biochemical markers of skeletal muscles and hepatic metabolism in men handball players. Methods: The present study was a quasi-experimental research method in which 12 handball players with at least 2 years' experience in a superior league (age= 21.42±1.56 years, height= 186 ± 5.85 cm and weight= 83.25 ± 10 kg, body mass index= 24.09 ± 2.93 kg. m-2) were randomly selected. Fasting blood samples were collected before and immediately after the maximum Bruce protocol test in order to measure the biochemical changes in skeletal muscles (LDH, CPK and lactate) and hepatic metabolism (ALT and AST). Paired t-test was used to analyze the data. The significance level was less than 0.05. Results: The results showed that the lactate dehydrogenase (P = 0.004), lactate (P = 0.001) and alanine aminotransferase (P = 0.001) levels increased significantly immediately after the exhaustive exercise. The levels of creatine phosphokinase (P = 0.20), aspartate aminotransferase (P = 0.16) increased but the increase was not statistically significant. Conclusion: According to the findings of this study, it can be concluded that progressive exhaustive exercise may increase in some of the biochemical markers of skeletal muscle and liver metabolism. Therefore, it is recommended to trainers and athletes to pay more attention to the training principles of practice.

Keywords


1. Cheung K, Hume PA, Maxwell L. Delayed onset muscle soreness. Sports Med. 2003; 33 (2): 145- 164.
2. Delcour L, Dallaudière B, Omoumi P, Kirchgesner T, Berg BV, Cyteval C, et al. Delayed onset muscle soreness. J Belgian Society Rad. 2014; 97 (5): 1- 8.
3. Schoenfeld BJ. Does exercise-induced muscle damage play a role in skeletal muscle hypertrophy?. J Strength Cond Res. 2012; 26 (5): 1441- 1453.
4. Khansooz M, Abedi B, Moradi M, Mehranpour A. Acute effects of on javdar supplementation on asparate aminotransferase (AST) and alanine aminotransferase (ALT) after exhaustive incremental exercise in men’s handball. Arak Univ Med Sci J. 2017; 19 (12): 61- 69.
5. Nishii T, Umemura Y, Kitagawa K. Full suspension mountain bike improves off-road cycling performance. J Sports Med Phys Fitness. 2004; 44 (4): 356.
6. Gowda S, Desai PB, Hull VV, Math AAK, Vernekar SN, Kulkarni SS. A review on laboratory liver function tests. Pan African Med J. 2009; 3: 1- 7.
7. Pettersson J, Hindorf U, Persson P, Bengtsson T, Malmqvist U, Werkström V, et al. Muscular exercise can cause highly pathological liver function tests in healthy men. Br J Clin Pharm. 2008; 65 (2): 253- 259.
8. Smith J, Garbutt G, Lopes P, Pedoe DT. Effects of prolonged strenuous exercise (marathon running) on biochemical and haematological markers used in the investigation of patients in the emergency department. Br J Sports Med. 2004; 38 (3): 292- 294.
9. Matsuse H, Shiba N, Umezu Y, Nago T, Maeda T, Tagawa Y, et al. Effects of a hybrid exercise on the activities of myogenic enzymes in plasma. Kurume Med J. 2006; 53 (3+ 4): 47- 51.
10. Pearcey GE, Bradbury-Squires DJ, Kawamoto J-E, Drinkwater EJ, Behm DG, Button DC. Foam rolling for delayed-onset muscle soreness and recovery of dynamic performance measures. J Athletic Training. 2015; 50 (1): 5- 13.
11. Parikh D, Ramanathan N. Exercise induced serum enzyme changes in untrained subjects. Indian J Physiol Pharm. 1977; 21 (3): 175- 180.
12. Bashiri J, Hadi H, Bashiri M, Nikbakht H, Gaeini A. Effect of concurrent creatine monohydrate ingestion and resistance training on hepatic enzymes activity levels in non-athlete males. Iranian J Endocrin Metab. 2010; 12 (1): 42- 47.
13. Jackson AS, Pollock ML. Generalized equations for predicting body density of men. Br J Nutr. 1978; 40 (3): 497- 504.
14. Siri W. Body composition from fluid spaces and density: analysis of methods. Nutr. 1993; 9 (5): 480.
15. Mahan L, Escott-Stump S. Macronutrients: Carbohydrates, proteins, and lipids” by: susan etlinger. Med Nutr Ther. 2004.
16. Medicine ACoS. ACSM's guidelines for exercise testing and prescription: Lippincott Williams & Wilkins. 2013.
17. Shin KA, Park KD, Ahn J, Park Y, Kim Y-J. Comparison of changes in biochemical markers for skeletal muscles, hepatic metabolism, and renal function after three types of long-distance running: observational study. Med J. 2016; 95 (20): 8- 14.
18. Baird MF, Graham SM, Baker JS, Bickerstaff GF. Creatine-kinase-and exercise-related muscle damage implications for muscle performance and recovery. J Nutr Metab. 2012; 2012.
19. Saengsirisuwan V, Phadungkij S, Pholpramool C. Renal and liver functions and muscle injuries during training and after competition in Thai boxers. Br J Sports Med. 1998; 32 (4): 304- 308.
20. Burgomaster KA, Hughes SC, Heigenhauser GJ, Bradwell SN, Gibala MJ. Six sessions of sprint interval training increases muscle oxidative potential and cycle endurance capacity in humans. J Appl Physiol. 2005; 98 (6): 1985- 1990.
21. Shoemaker J, Phillips S, Green H, Hughson R. Faster femoral artery blood velocity kinetics at the onset of exercise following short-term training. Cardiovas Res. 1996; 31 (2): 278- 286.
22. Green H, Barr D, Fowles J, Sandiford S, Ouyang J. Malleability of human skeletal muscle Na+-K+-ATPase pump with short-term training. J Appl Physiol. 2004; 97 (1): 143- 148.
23. Illera J, Silvan G, Lorenzo P, Portela A, Illera M, Illera M. Photoperiod variations
of various blood biochemistry constants in the rabbit. Rev Espanola de Fisiologia. 1992; 48 (1): 7- 12.
24. Barzegarzadeh- Zarandi H, Dabidy- Roshan V. Changes in some liver enzymes and blood lipid level following interval and continuous regular aerobic training in old rats. J Shahrekord Uuni Med Sci J. 2012; 14: 12- 18.
25. Clarkson PM, Hubal MJ. Exercise- induced muscle damage in humans. Am J Phys Med Rehab. 2002; 81 (11): S52- S69.
26. Rezaei M, Rahimi E, Bordbar S, Namdar S. The effects of three sessions of running on a negative slope on serum levels of liver enzymes in adult male rats. Zahedan J Res Med Sci. 2013; 15 (5): 47- 49.
27. Zar A, Hosseini SA, Homaion A. Effect of eight- week aquagymnastic training on liver enzymes and lipid profile of middle- aged women. Qum Med Sci Univ J. 2016; 10 (7): 29- 37.
28. Cinar K, Coban S, Idilman R, Tuzun A, Sarioglu M, Bektas M, et al. Long‐term prognosis of nonalcoholic fatty liver disease: Is pharmacological therapy actually necessary?. J Aastroenterology Hepatology. 2006; 21 (1): 169- 173.
29. Masoodsinaki H, Nazarali P, Hanachi P. Evaluation and impact of omega-3 supplementation with a period of selective aerobic exercise on liver enzymes (AST-ALT) of active student girls. Hormozgan Uni Med Sci J. 2014; 18 (3): 226- 234.